JEOL JEM 1400 Transmission Electron Microscope
The JEOL JEM-1400 is a highly versatile 120 kV transmission electron microscope that provides high-quality ultrastructural imaging across a broad range of biological and materials science applications. As the Bioimaging Centre's principal platform for conventional TEM, the system supports routine and advanced investigations of cells, tissues, microorganisms, biomaterials and nanoparticles, delivering detailed insights into morphology and cellular architecture at the nanometre scale.
The microscope is ideally suited for the examination of resin-embedded specimens, ultrathin sections, negatively stained samples and immunogold-labelled preparations. Researchers use the system to investigate cellular ultrastructure, organelle organisation, host-pathogen interactions, tissue morphology, extracellular vesicles, nanomaterials and a wide variety of biological and biomedical questions. The platform is routinely used for quantitative ultrastructural analysis, enabling statistically robust measurements of organelles, cellular compartments, nanoparticles and tissue architecture through a range of morphometric and stereological approaches.
Image acquisition is provided by a Gatan Rio16 4k × 4k digital camera, delivering high-resolution digital imaging with excellent sensitivity and a large field of view. Combined with the microscope's stability, ease of operation and broad application range, the system is equally suited to specimen screening, quantitative analysis and publication-quality image acquisition.
Supported by our experienced electron microscopy specialists, the JEM-1400 provides researchers with comprehensive training, technical expertise and experimental guidance, making it an ideal platform for routine ultrastructural investigations, method development, teaching and advanced transmission electron microscopy studies across the life and physical sciences.
Key Features
- 120 kV transmission electron microscope
- Gatan Rio16 4k × 4k digital camera
- Magnification range from 200× to 1,000,000×
- High-resolution imaging with a lattice resolution of 0.2 nm
- Conventional TEM imaging of biological and materials specimens
- Negative stain TEM
- Resin-embedded specimen imaging
- Immunogold labelling and localisation studies
- Nanoparticle and extracellular vesicle characterisation
- Quantitative morphometric and stereological analysis workflows
- Large-area digital image acquisition
- Training and specialist user support
Technical Specifications
| Specification | Details |
|---|---|
| Microscope | JEOL JEM-1400 |
| Accelerating Voltage | 120 kV |
| Electron Source | Tungsten filament |
| Camera | Gatan Rio16, 4k × 4k CCD |
| Magnification Range | 200× to 1,000,000× |
| Lattice Resolution | 0.2 nm |
| Applications | Conventional TEM, negative stain TEM, immunogold labelling, nanoparticle analysis, morphometrics |
Typical Applications
- Cellular and tissue ultrastructure
- Organelle morphology and quantification
- Host-pathogen interactions
- Immunogold localisation studies
- Nanoparticle and nanomaterial characterisation
- Extracellular vesicle imaging
- Biological and biomedical research
- Quantitative morphometric analysis
- Negative stain imaging of macromolecular complexes - quality control
- Teaching, training and method development
This system continues to serve as the Bioimaging Centre's core platform for conventional transmission electron microscopy, providing reliable, high-quality ultrastructural imaging and quantitative analysis capabilities across a diverse range of research disciplines.
